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Modulation of induced reversion frequency by nucleotide pool imbalance as a tool for mutant characterization
Author(s) -
Randazzo Rosalba,
di Leonardo A.,
Bonatti Stefania,
Abbondandolo A.
Publication year - 1987
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850100104
Subject(s) - reversion , mutant , chinese hamster , mutagen , methyl methanesulfonate , ethyl methanesulfonate , mutation frequency , hypoxanthine guanine phosphoribosyltransferase , mutation , hypoxanthine , guanine , biology , microbiology and biotechnology , biochemistry , chemistry , genetics , nucleotide , dna , phenotype , enzyme , gene
Addition of thymidine (TdR) or deoxycytine (CdR) to the culture medium during posttreatment incubation affected the frequency of mutagen‐induced reversion in three hypoxanthine‐guanine phosphoribosyl transferase‐deficient mutants of V79 Chinese hamster cells. With two of the mutants (E20 and 13), reversions induced by N‐ethylnitrosourea, ethyl methanesulfonate, and methyl methanesulfonate were enhanced by TdR and were either decreased (E20) or not affected (13) by CdR. With the third mutant (E21), alkylating agent‐induced reversions were enhanced by CdR and decreased by TdR. Finally, 6‐amino‐2‐hydroxypurine induced reversions were enhanced by TdR in mutant I3 and were decreased by TdR or deoxyadenosine (AdR) and enhanced by CdR in mutant E21. An attempt was made to reconcile these results with simple mutation mechanisms, based on either G:C to A:T or A:T to G:C transitions. It is suggested that the present approach may add useful information to studies of specific revertibility of mammalian cell mutants with known mutagens.