Premium
Effect of dose and dose rate of gamma irradiation on the formation of micronuclei in bone marrow cells isolated from whole‐body‐irradiated mice
Author(s) -
D'Auria Vieira de Godoy Paulo Roberto,
Nakamura Ayumi,
Khavari Ali Pour,
Sangsuwan Traimate,
Haghdoost Siamak
Publication year - 2021
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.22453
Subject(s) - micronucleus test , oxidative stress , ionizing radiation , dna damage , reactive oxygen species , irradiation , bone marrow , chemistry , in vitro , micronucleus , dose–response relationship , cytotoxicity , toxicology , radiochemistry , toxicity , dna , pharmacology , immunology , biology , biochemistry , physics , organic chemistry , nuclear physics
It is well‐known that the cytotoxicity and mutagenic effects of high dose rate (HDR) ionizing radiation (IR) are increased by increasing the dose but less is known about the effects of chronic low dose rate (LDR). In vitro, we have shown that in addition to the immediate interaction of IR with DNA (the direct and indirect effects), low doses and chronic LDR exposure induce endogenous oxidative stress. During elevated oxidative stress, reactive oxygen species (ROS) react with DNA modifying its structure. Here, BL6 mice were exposed to IR at LDR and HDR and were then sacrificed 3 hours and 3 weeks after exposure to examine early and late effects of IR. The levels of micronuclei, MN, were determined in bone marrow cells. Our data indicate that the effects of 200 mGy on MN‐induction are transient, but 500 and 1000 mGy (both HDR and LDR) lead to increased levels of MN up to 3 weeks after the exposure.