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Mutational spectra induced by flavonoid extracts from pepper tree ( Schinus terebinthifolius , Raddi) stem bark
Author(s) -
VarelaBarca Francisco Napoleão Tulio,
Felipe Maria Beatriz Mesquita Cansanção,
Medeiros Sílvia Regina Batistuzzo
Publication year - 2021
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.22415
Subject(s) - flavonoid , biology , pepper , escherichia coli , bark (sound) , dna damage , mutagenesis , oxidative stress , mutation , dna , biochemistry , gene , antioxidant , food science , ecology
Abstract Flavonoids are a diverse family of plant compounds that are involved in pigmentation, protection, and endogenous regulation. Flavonoids also have medicinal applications, suggesting that they may exert chemoprotective effects. However, some studies have shown, that some plant flavonoids have oxidative and toxic effects, including those produced by Schinus terebinthifolius . In Brazil, extracts of this plant are widely used for medical purposes. In this study, we analyzed the mutagenic potential of two flavonoid‐enriched fractions from Brazilian pepper tree stem bark using Escherichia coli CC strains deficient and proficient in enzymes involved in the DNA repair of oxidative lesions. The highest mutagenic response was detected in the CC104 mut M mut Y strain but CC104 mut Y showed a higher mutation frequency than CC104 mut M . The spectrum of mutations induced in plasmid DNA is composed of mutations typically caused by oxidative lesions. However, a new type of lesion must be occurred to explain the cytotoxicity, higher mutation rates in the CC104 mut Y strain, and the rare A:T → T:A and G:C → C:G transversions found in this work.