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Radio‐adaptive response, individual radio‐sensitivity and correlation of base excision repair gene polymorphism ( hOGG1 , APE1 , XRCC1, and LIGASE1 ) in human peripheral blood mononuclear cells exposed to gamma radiation
Author(s) -
Toprani Sneh M.,
Das Birajalaxmi
Publication year - 2020
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.22383
Subject(s) - xrcc1 , base excision repair , dna repair , single nucleotide polymorphism , biology , dna damage , gene , nucleotide excision repair , microbiology and biotechnology , genotype , genetics , dna
Abstract Radio‐adaptive response (RAR) is a biological mechanism, where cells primed with a low dose exhibit reduced DNA damage with a high challenging dose. Single nucleotide polymorphisms (SNPs) of DNA repair genes including base excision repair (BER) pathway are known to be associated with radio‐sensitivity but involvement in RAR is not yet understood. In the present study, attempt was made to correlate genotype frequencies of four BER SNPs [ hOGG1 (Ser326Cys), XRCC1 (Arg399Gln), APE1 (Asp148Glu) and LIGASE1 (A/C)] with DNA damage, repair and mRNA expression level among 20 healthy donors (12 adaptive and 8 nonadaptive). Our results revealed that LIGASE1 ( p = .002) showed significant correlation with DNA damage and mRNA expression level with increasing dose. hOGG1 (Ser326Cys), XRCC1 (Arg399Gln) and LIGASE1 (A/C) polymorphisms showed significant difference with DNA damage (%T) and mRNA expression profile in primed cells among adaptive donors. In conclusion, BER gene polymorphisms play important role in identifying donors with radio‐sensitivity and RAR in human cells.