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The rs6983267 SNP and long non‐coding RNA CARLo‐5 are associated with endometrial carcinoma
Author(s) -
Zhao Xiwa,
Wei Xurui,
Zhao Lianmei,
Shi Li,
Cheng Jianxin,
Kang Shan,
Zhang Hui,
Zhang Jun,
Li Li,
Zhang Haibo,
Zhao Wei
Publication year - 2016
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.22031
Subject(s) - snp , genotype , biology , single nucleotide polymorphism , long non coding rna , oncology , genetics , rna , gene , medicine
The single nucleotide polymorphism (SNP) rs6983267 and cancer‐associated region long non‐coding RNA ( CARLo‐5 ) are associated with various human cancers. This study aimed to investigate the expression of CARLo‐5 in endometrial carcinoma (EC) and its relationship with clinicopathological features and patient survival. The association of the rs6983267 SNP with EC risk and its involvement in the regulation of CARLo‐5 expression in EC were investigated. The rs6983267 SNP was genotyped by polymerase chain reaction (PCR) and ligase detection reaction in 543 EC patients and 584 controls. The expression of CARLo‐5 in 108 EC tissues and 66 normal endometrial tissues (NETs) was determined using quantitative real‐time PCR. The genotype and allele distributions of the rs6983267 SNP differed significantly between patients and controls. There was a significant correlation between the rs6983267 genotypes and lymph node metastasis of EC patients ( P = 0.026). CARLo‐5 expression was significantly higher in EC tissues than in NETs ( P < 0.001) and significantly associated with FIGO stage ( P = 0.029) and lymph node metastasis ( P = 0.030). Patients with high CARLo‐5 expression had significantly shorter overall survival than those with low CARLo‐5 expression ( P = 0.003). The rs6983267 genotype was significantly correlated with CARLo‐5 expression ( P < 0.05). In conclusion, CARLo‐5 was identified as a pro‐oncogenic lncRNA that may play an important role in EC progression and represent a prognostic marker for EC. The expression of CARLo‐5 was significantly correlated with the rs6983267 genotype associated with increased susceptibility to EC. Environ. Mol. Mutagen. 57:508–515, 2016. © 2016 Wiley Periodicals, Inc.