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Biomarkers of oxidative stress and inflammation after wood smoke exposure in a reconstructed Viking Age house
Author(s) -
Jensen Annie,
Karottki Dorina Gabriela,
Christensen Jannie Marie,
Bønløkke Jakob Hjort,
Sigsgaard Torben,
Glasius Marianne,
Loft Steffen,
Møller Peter
Publication year - 2014
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.21877
Subject(s) - oxidative stress , dna damage , inflammation , comet assay , hmox1 , genotoxicity , chemistry , andrology , dna glycosylase , inhalation exposure , systemic inflammation , immunology , endocrinology , microbiology and biotechnology , medicine , biology , biochemistry , dna , toxicity , enzyme , heme , heme oxygenase
Exposure to particles from combustion of wood is associated with respiratory symptoms, whereas there is limited knowledge about systemic effects. We investigated effects on systemic inflammation, oxidative stress and DNA damage in humans who lived in a reconstructed Viking Age house, with indoor combustion of wood for heating and cooking. The subjects were exposed to high indoor concentrations of PM 2.5 (700–3,600 µg/m 3 ), CO (10.7–15.3 ppm) and NO 2 (140–154 µg/m 3 ) during a 1‐week stay. Nevertheless, there were unaltered levels of genotoxicity, determined as DNA strand breaks and formamidopyrimidine DNA glycosylase and oxoguanine DNA glycosylase 1 sensitive sites in peripheral blood mononuclear cells. There were also unaltered expression levels of OGG1 , HMOX1 , CCL2 , IL8 , and TNF levels in leukocytes. In serum, there were unaltered levels of C‐reactive protein, IL6, IL8, TNF, lactate dehydrogenase, cholesterol, triglycerides, and high‐density lipoproteins. The wood smoke exposure was associated with decreased serum levels of sICAM‐1, and a tendency to decreased sVCAM‐1 levels. There was a minor increase in the levels of circulating monocytes expressing CD31, whereas there were unaltered expression levels of CD11b, CD49d, and CD62L on monocytes after the stay in the house. In conclusion, even a high inhalation exposure to wood smoke was associated with limited systemic effects on markers of oxidative stress, DNA damage, inflammation, and monocyte activation. Environ. Mol. Mutagen. 55:652–661, 2014. © 2014 Wiley Periodicals, Inc.