Premium
Effects of short‐term exposure to inhalable particulate matter on DNA methylation of tandem repeats
Author(s) -
Guo Liqiong,
Byun HyangMin,
Zhong Jia,
Motta Valeria,
Barupal Jitendra,
Zheng Yinan,
Dou Chang,
Zhang Feiruo,
McCracken John P.,
Diaz Anaité,
Marco SanchezGuerra,
Colicino Silvia,
Schwartz Joel,
Wang Sheng,
Hou Lifang,
Baccarelli Andrea A.
Publication year - 2014
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.21838
Subject(s) - dna methylation , methylation , particulates , chemistry , zoology , biology , genetics , dna , ecology , gene , gene expression
There is compelling evidence that particulate matter (PM) increases lung cancer risk by triggering systemic inflammation, and leukocyte DNA hypomethylation. However, previous investigations focused on repeated element sequences from LINE‐1 and Alu families. Tandem repeats, which display a greater propensity to mutate, and are often hypomethylated in cancer patients, have never been investigated in individuals exposed to PM. We measured methylation of three tandem repeats ( SATα , NBL2 , and D4Z4 ) by polymerase chain reaction–pyrosequencing on blood samples from truck drivers and office workers (60 per group) in Beijing, China. We used lightweight monitors to measure personal PM 2.5 (PM with aerodynamic diameter ≤2.5 µm) and elemental carbon (a tracer of PM from vehicular traffic). Ambient PM 10 data were obtained from air quality measuring stations. Overall, an interquartile increase in personal PM 2.5 and ambient PM 10 levels was associated with a significant covariate‐adjusted decrease in SATα methylation (−1.35% 5‐methyl cytosine [5mC], P = 0.01; and −1.33%5mC; P = 0.01, respectively). Effects from personal PM 2.5 and ambient PM 10 on SATα methylation were stronger in truck drivers (−2.34%5mC, P = 0.02; −1.44%5mC, P = 0.06) than office workers (−0.95%5mC, P = 0.26; −1.25%5mC, P = 0.12, respectively). Ambient PM 10 was negatively correlated with NBL2 methylation in truck drivers (−1.38%5mC, P = 0.03) but not in office workers (1.04%5mC, P = 0.13). Our result suggests that PM exposure is associated with hypomethylation of selected tandem repeats. Measuring tandem‐repeat hypomethylation in easy‐to‐obtain blood specimens might identify individuals with biological effects and potential cancer risk from PM exposure. Environ. Mol. Mutagen. 55:322–335, 2014. © 2014 Wiley Periodicals, Inc.