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AFB 1 ‐induced mutagenesis of the gpt gene in AS52 cells
Author(s) -
Wattanawaraporn Roongtiwa,
Kim Min Young,
Adams Jillian,
Trudel Laura J.,
Woo Leslie L.,
Croy Robert G.,
Essigmann John M.,
Wogan Gerald N.
Publication year - 2012
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.21711
Subject(s) - mutagen , mutagenesis , biology , gene , aflatoxin , genetics , microbiology and biotechnology , transgene , mutation frequency , carcinogen , hepatocellular carcinoma , mutation
Aflatoxin B 1 (AFB 1 ) is a potent mutagen and an important risk factor for hepatocellular carcinoma (HCC) in humans. Transgenic mouse strains and cells in culture have been used to detect different types of mutations caused by AFB 1 and investigate the molecular determinants of their location and frequency. The AFB 1 mutational spectrum in the gpt gene was markedly different in AS52 cells compared with the liver in gpt delta B6C3F1 transgenic mice. The results demonstrate the importance of metabolism, chromosomal location, transcription and selection conditions on mutational spectra. Environ. Mol. Mutagen. 2012. © 2012 Wiley Periodicals, Inc.