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Mouse lymphoma thymidine kinase gene mutation assay: Follow‐up meeting of the international workshop on Genotoxicity testing—Aberdeen, Scotland, 2003—Assay acceptance criteria, positive controls, and data evaluation
Author(s) -
Moore Martha M.,
Honma Masamitsu,
Clements Julie,
Bolcsfoldi George,
Burlinson Brian,
Cifone Maria,
Clarke Jane,
Delongchamp Robert,
Durward Robert,
Fellows Michael,
Gollapudi Bhaskar,
Hou Saimei,
Jenkinson Peter,
Lloyd Melvin,
Majeska Jenness,
Myhr Brian,
O'Donovan Michael,
Omori Takashi,
Riach Colin,
San Richard,
Stankowski Leon F.,
Thakur Ajit K.,
Van Goethem Freddy,
Wakuri Shinobu,
Yoshimura Isao
Publication year - 2006
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.20159
Subject(s) - workgroup , genotoxicity , biology , genetics , medicine , computer science , toxicity , computer network
Abstract The Mouse Lymphoma Assay (MLA) Workgroup of the International Workshop on Genotoxicity Testing (IWGT), comprised of experts from Japan, Europe, and the United States, met on August 29, 2003, in Aberdeen, Scotland, United Kingdom. This meeting of the MLA Workgroup was devoted to reaching a consensus on the appropriate approach to data evaluation and on acceptance criteria for both the positive and negative/vehicle controls. The Workgroup reached consensus on the acceptance criteria for both the agar and microwell versions of the MLA. Recommendations include acceptable ranges for mutant frequency, cloning efficiency, and suspension growth of the negative/vehicle controls and on criteria to define an acceptable positive control response. The recommendation for the determination of a positive/negative test chemical response includes both the requirement that the response exceeds a defined value [the global evaluation factor (GEF)] and that there also be a positive dose–response (evaluated by an appropriate statistical method). Environ. Mol. Mutagen., 2006. Published 2005 Wiley‐Liss, Inc.