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Evaluation of the Salmonella umu test with 83 NTP chemicals
Author(s) -
Yasunaga Katsuaki,
Kiyonari Akiko,
Oikawa Takeshi,
Abe Naoki,
Yoshikawa Kunie
Publication year - 2004
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.20053
Subject(s) - salmonella , genotoxicity , carcinogen , ames test , enterobacteriaceae , mutagen , chemistry , concordance , bioassay , toxicology , food science , biology , microbiology and biotechnology , gene , bacteria , toxicity , biochemistry , genetics , escherichia coli , organic chemistry
There is a need for simple rapid tests for evaluating the carcinogenic potential of the thousands of chemical compounds that are developed each year. The DNA‐damaging effects of 83 National Toxicology Program (NTP) chemicals, including noncarcinogens and carcinogens, were examined in the umu test by measuring the expression of the umuDC‐lacZ genes in Salmonella typhimurium TA1535/pSK1002. Salmonella were exposed to individual NTP chemicals at 37°C for 2 hr both with and without a rat liver S9 mix; the treated cells were then diluted and incubated for a further 2 hr (posttreatment assay). O ‐nitrophenyl‐β‐ D ‐galactoside was added to the cultures and the β‐galactosidase activity driven by the Salmonella umuDC‐lacZ genes was determined by measurement of the OD 420 nm and OD 550 nm of the cultures. Salmonella cell number was simultaneously determined by measurement of OD 600 nm . The overall concordance between genotoxicity in the umu test and carcinogenicity was 67%, which was similar to the concordance between Ames' test results and carcinogenicity (63%) using the same 83 NTP chemicals. The results of this study indicate that the umu test with a single Salmonella strain is a simple rapid system, with accuracy comparable to existing, more time‐consuming assays. Environ. Mol. Mutagen. 44:329–345, 2004. © 2004 Wiley‐Liss, Inc.