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DNA damage produced in HaCaT cells by combined fluoranthene exposure and ultraviolet A irradiation
Author(s) -
Zheng Baoying,
Hwang HueyMin,
Yu Hongtao,
Ekunwe Stephen
Publication year - 2004
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.20040
Subject(s) - hacat , fluoranthene , cytotoxicity , genotoxicity , dna damage , comet assay , chemistry , toxicity , carcinogen , microbiology and biotechnology , dna , biology , toxicology , biochemistry , environmental chemistry , in vitro , phenanthrene , organic chemistry
Fluoranthene is a polycyclic aromatic hydrocarbon (PAH) and a principal constituent of PAH‐contaminated aquatic systems. In the present study, fluorescein diacetate uptake and the Comet assay were used to assess the cytotoxicity and genotoxicity of fluoranthene in HaCaT (human adult low calcium high temperature) cells in the presence or absence of ultraviolet A (UVA) irradiation. Exposure of cells to 0.1, 0.25, 0.75, 2, and 5 μM fluoranthene alone for 30 min or to 6.1 ± 0.07 J/cm 2 UVA alone did not cause cytotoxicity or cellular DNA damage. However, concomitant exposure to both caused a nonlinear dose‐response in cytotoxicity to HaCat cells. The same exposure conditions also resulted in a dose‐responsive DNA damage in HaCaT cells. Because DNA damage mainly was detected at relatively high levels of cytotoxicity, we cannot rule out the possibility that it occurred as a consequence of cellular toxicity mechanisms. Environ. Mol. Mutagen. 44:151–155, 2004. © 2004 Wiley‐Liss, Inc.