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DNA adducts of 1,3‐butadiene in humans: Relationships to exposure, GST genotypes, single‐strand breaks, and cytogenetic end points
Author(s) -
Zhao Chunyan,
Vodicka Pavel,
Sram Radim J.,
Hemminki Kari
Publication year - 2001
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.1031
Subject(s) - genotype , glutathione , adduct , dna adduct , dna damage , dna , biology , microbiology and biotechnology , medicine , genetics , endocrinology , chemistry , biochemistry , enzyme , gene , organic chemistry
The modulation of 1,3‐butadiene (BD)‐induced DNA adducts by occupational exposure, glutathione S ‐transferase (GST) genotypes, single‐strand breaks, and cytogenetic end points was studied in 15 workers and 11 controls. The exposed group consisted of 8 smokers and 7 nonsmokers, whereas the control group consisted of 7 nonsmokers and 4 smokers. Among all subjects, the adduct levels in workers lacking GSTM1 were significantly higher than in those who were GSTM1 positive ( P = 0.026), and individuals with combined GSTM1(−) and GSTT1(+) genotype had elevated level of adducts compared to that of persons with GSTM1(+) and GSTT1(+) ( P = 0.049). The increase in BD–DNA adduct levels in all subjects was significantly related to BD exposure and GSTM1 genotype (linear multiple regression analysis, P = 0.001; P = 0.035). The results suggest that DNA adducts serve as a sensitive and specific biomarker, integrating exposure and host metabolic capacity, although the data are limited to a small number of subjects. Environ. Mol. Mutagen. 37:226–230, 2001. © 2001 Wiley‐Liss, Inc.