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Treatment and sampling protocols for transgenic mutation assays
Author(s) -
Heddle John A.,
Martus HansJoerg,
Douglas George R.
Publication year - 2003
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.10131
Subject(s) - mutation , sampling (signal processing) , mutation frequency , transgene , biology , mutant , mutagenesis , computational biology , sampling time , genetics , computer science , statistics , gene , mathematics , filter (signal processing) , computer vision
The standard protocol for testing chemicals with the transgenic mutation assays in vivo includes a period of time between treatment and sampling to permit the mutation frequency to reach a maximum. Recent evidence has shown, however, that for some chemicals the mutant frequency can decline substantially during this period, which would reduce the sensitivity of the assay. Here we discuss alternate protocols to maintain the sensitivity of the assay for both types of mutagens and, in particular, propose that treatments should continue until the time of sampling. Environ. Mol. Mutagen. 41:1–6, 2003. © 2003 Wiley‐Liss, Inc.