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Mouse lymphoma thymidine kinase gene mutation assay: Follow‐up International Workshop on Genotoxicity Test Procedures, New Orleans, Louisiana, April 2000
Author(s) -
Moore Martha M.,
Honma Masamitsu,
Clements Julie,
HarringtonBrock Karen,
Awogi Takumi,
Bolcsfoldi George,
Cifone Maria,
Collard Deborah,
Fellows Michael,
Flanders Kathryn,
Gollapudi Bhaskar,
Jenkinson Peter,
Kirby Paul,
Kirchner Stephan,
Kraycer Joann,
McEnaney Stephen,
Muster Wolfgang,
Myhr Brian,
O'Donovan Michael,
Oliver Jo,
Ouldelhkim MarieClaude,
Pant Kamala,
Preston Robert,
Riach Colin,
San Richard,
Shimada Hiroyasu,
Stankowski Leon F.
Publication year - 2002
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.10122
Subject(s) - workgroup , genotoxicity , cytotoxicity , biology , computer science , medicine , genetics , toxicity , computer network , in vitro
The Mouse Lymphoma Assay (MLA) Workgroup of the International Workshop on Genotoxicity Test Procedures held a second harmonization meeting just prior to the U.S. Environmental Mutagen Society Meeting in New Orleans, LA, in April 2000. The discussion focused on several important aspects of the MLA, including: 1) cytotoxicity measures and their determination, 2) use of a 24‐hr treatment, 3) the ability of the assay to detect aneugens, and 4) concentration selection. Prior to the meeting the group developed Microsoft Excel Workbooks for data entry. Ten laboratories entered their data into the workbooks (primarily as coded chemicals). The Excel Workbooks were used to facilitate data analysis by generating an extensive set of graphs that were evaluated by the meeting participants. Based on the Workgroup's previous agreement that a single cytotoxicity measure should be established for both the microwell and soft agar versions of the assay, the Workgroup analyzed the submitted data and unanimously agreed that the relative total growth (RTG) should be used as the cytotoxicity measure for concentration selection and data evaluation. The Workgroup also agreed that the various cytotoxicity measures should be calculated using the same methods regardless of whether the soft agar or microwell version of the assay was used. In the absence of sufficient data to make a definitive determination, the Workgroup continued to endorse the International Committee on Harmonization recommendation for the use of 24‐hr treatment and made some specific 24‐hr treatment protocol recommendations. The Workgroup recognized the ability of the MLA to detect at least some aneugens and also developed general guidance and requirements for appropriate concentration selection. Environ. Mol. Mutagen. 40:292–299, 2002. © 2002 Wiley‐Liss, Inc.