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Assessment of DNA damage in lymphocytes of workers exposed to X‐radiation using the micronucleus test and the comet assay
Author(s) -
Maluf Sharbel Weidner,
Passos Daniela Ferreira,
Bacelar Alexandre,
Speit Günter,
Erdtmann Bernardo
Publication year - 2001
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.10029
Subject(s) - micronucleus test , comet assay , clastogen , dicentric chromosome , micronucleus , dna damage , mutagen , genotoxicity , toxicology , biology , genetics , microbiology and biotechnology , carcinogen , toxicity , dna , medicine , gene , karyotype , chromosome
The mutagenic and carcinogenic effects of genotoxic agents on exposed people have constituted an increasing concern. Therefore, the objective of this work was to assess DNA damage in lymphocytes of workers exposed to X‐radiation using the cytokinesis‐blocked micronucleus test and the comet assay (single‐cell gel electrophoresis), and to compare these two techniques in the monitoring of exposed populations. The cytokinesis‐blocked micronucleus test and the comet assay were employed in the monitoring of 22 workers occupationally exposed to X‐radiation in a hospital in southern Brazil. The frequency of dicentric bridges was also measured. The results of both assays and the frequency of dicentric bridges revealed a significant increase in genetic effects on the cells of exposed individuals. Age was significantly correlated with micronucleus frequency and damage index in the comet assay. The concomitant analysis of dicentric bridges when determining micronucleus frequency does not require much extra work, and may serve as a reference to the type of mutagenic effect (clastogenic or aneugenic). The combination of the alkaline comet assay with the cytokinesis‐blocked micronucleus test appears to be very informative for the monitoring of populations chronically exposed to genotoxic agents. Environ. Mol. Mutagen. 38:311–315, 2001. © 2001 Wiley‐Liss, Inc.