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Genotoxicity of some sulfur dyes on tadpoles ( Rana hexadactyla ) measured using the comet assay
Author(s) -
Rajaguru P.,
Kalpana R.,
Hema A.,
Suba S.,
Baskarasethupathi B.,
Kumar P. A.,
Kalaiselvi K.
Publication year - 2001
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.10027
Subject(s) - genotoxicity , comet assay , dna damage , gel electrophoresis , dna , biology , chemistry , toxicology , microbiology and biotechnology , toxicity , biochemistry , organic chemistry
This report presents the results of a genotoxicity study to evaluate the DNA damage caused by four sulfur dyes used in the textile and tannery industries. Alkaline single‐cell gel electrophoresis assay (SCGE) was performed on erythrocytes from Rana hexadactyla tadpoles following whole‐body exposure to increasing concentrations of the dyes. The dyes, along with their active ingredients, were Sandopel Basic Black BHLN, Negrosine, Dermapel Black FNI, and Turquoise Blue. The dye‐treated tadpoles showed significant DNA damage, measured as mean DNA length:width ratio, when compared with unexposed control animals. Among the four tested dyes Sandopel Basic Black BHLN appears to be highly genotoxic, Dermapel Black FNI was least genotoxic, and Negrosine and Turquoise Blue were moderately toxic to R. hexadactyla tadpoles. The tadpoles showed a significant reduction in DNA damage when placed in dechlorinated tap water after exposure for a 24‐hr period to the dye solutions. Environ. Mol. Mutagen. 38:316–322, 2001. © 2001 Wiley‐Liss, Inc.