Open AccessA novel approach for enumeration of extracellular vesicles from crude and purified cell culture samples
Author(s) -
Kruse Thomas,
Schneider Samuel,
Reger Lucas Nik,
Kampmann Markus,
Reif OscarWerner
Publication year - 2022
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.202100149
Subject(s) - extracellular vesicles , enumeration , flow cytometry , biopharmaceutical , zeta potential , dynamic light scattering , chromatography , vesicle , chemistry , nanotechnology , size exclusion chromatography , computational biology , biology , membrane , biochemistry , microbiology and biotechnology , materials science , mathematics , nanoparticle , combinatorics , enzyme
The interest in extracellular vesicles (EVs) has been increased in recent years due to their potential application in diagnosis and therapy of severe diseases. The versatile fields of application due to the numerous possible cargos and the targeted delivery system make them a promising biopharmaceutical product. However, their broad size range as well as varied surface protein content result in challenges for the purification, characterization, and quantification. In this study a novel method, based on high‐resolution flow cytometry, was examined for the enumeration of EVs in purified as well as crude process samples. In addition to quantification, samples were characterized by dynamic light scattering, zeta potential measurement, and analytical size exclusion chromatography. It has been demonstrated that EVs were successfully enumerated with the novel method, offering great benefits for development and monitoring of EV processes.