Biosynthesis of ( R )‐(+)‐perillyl alcohol by  Escherichia coli  expressing neryl pyrophosphate synthase | Zendy

Sun Chao | Zendy; Zhang Rubing | Zendy; Xie Congxia | Zendy

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Biosynthesis of ( R )‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase

Author(s) -

Sun Chao,

Zhang Rubing,

Xie Congxia

Publication year - 2022

Publication title -

engineering in life sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.547

H-Index - 57

eISSN - 1618-2863

pISSN - 1618-0240

DOI - 10.1002/elsc.202100135

Subject(s) - escherichia coli , alcohol , chemistry , metabolic engineering , biosynthesis , atp synthase , titer , biochemistry , pyrophosphate , enzyme , biology , antibody , immunology , gene

( R )‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of ( R )‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of ( R )‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration. Engineering neryl pyrophosphate (NPP)‐supplied pathway resulted in a 4‐fold improvement of ( R )‐(+)‐perillyl alcohol titer. Subsequently, combined engineering of p‐cymene monooxygenase ( CymA ) expression and module for NADPH regeneration exhibited a 15.4‐fold increase of titer over the initial strain S02. Finally, 453 mg/L ( R )‐(+)‐perillyl alcohol was achieved in fed‐batch fermentation, which is the highest ( R )‐(+)‐perillyl alcohol titer in E. coli .

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