Open AccessBiosynthesis of ( R )‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase
Author(s) -
Sun Chao,
Zhang Rubing,
Xie Congxia
Publication year - 2022
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.202100135
Subject(s) - escherichia coli , alcohol , chemistry , metabolic engineering , biosynthesis , atp synthase , titer , biochemistry , pyrophosphate , enzyme , biology , antibody , immunology , gene
( R )‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of ( R )‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of ( R )‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration. Engineering neryl pyrophosphate (NPP)‐supplied pathway resulted in a 4‐fold improvement of ( R )‐(+)‐perillyl alcohol titer. Subsequently, combined engineering of p‐cymene monooxygenase ( CymA ) expression and module for NADPH regeneration exhibited a 15.4‐fold increase of titer over the initial strain S02. Finally, 453 mg/L ( R )‐(+)‐perillyl alcohol was achieved in fed‐batch fermentation, which is the highest ( R )‐(+)‐perillyl alcohol titer in E. coli .