Open AccessScalable downstream method for the cyclic lipopetide jagaricin
Author(s) -
Stein Jakob,
Schlosser Nicolas,
Bardl Bettina,
Peschel Gundela,
Meyer Florian,
Kloss Florian,
Rosenbaum Miriam A.,
Regestein Lars
Publication year - 2022
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.202100079
Subject(s) - downstream processing , downstream (manufacturing) , derivatization , amphiphile , fractionation , chemistry , process (computing) , combinatorial chemistry , nanotechnology , biochemical engineering , process engineering , computer science , materials science , chromatography , organic chemistry , polymer , high performance liquid chromatography , engineering , operations management , copolymer , operating system
Cyclic lipopeptides are substances with a high potential to act as antimicrobial agents. Jagaricin, produced by Janthinobacterium agaricidamnosum DSM 9628 and discovered in 2012, is a new member of this class with promising antifungal properties. However, further experiments to investigate future applications and/or conduct chemical derivatization to change properties and toxicity are impossible due to the limited access to jagaricin. Besides a high jagaricin concentration at the end of the fermentation process, a suitable downstream process is essential to generate appropriate amounts with the desired purity. In contrast to other amphiphilic molecules, jagaricin cannot be separated by foam fractionation since it is mainly attached to the surface of the microbial biomass. This technical report presents an overall process chain consisting of 11 individual steps to generate jagaricin in gram scale with a purity of over 95%.