Open AccessYeast autonomously replicating sequence (ARS): Identification, function, and modification
Author(s) -
Tan XiaoYu,
Wu XiaoLe,
Han MingZhe,
Wang Li,
Xu Li,
Li BingZhi,
Yuan YingJin
Publication year - 2021
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.202000085
Subject(s) - biology , origin of replication , dna replication , autonomously replicating sequence , computational biology , genetics , control of chromosome duplication , chromosome , dna sequencing , gene
Eukaryotic DNA replication begins with multiple origins of replication (ORIs) and thus exploring and identifying ORIs is essential for further understanding of the DNA replication process. In budding yeast, certain autonomously replicating sequences (ARSs) initiate the replication as ORIs, maintaining the stability of chromosomes and plasmids during genome replication. Currently, ARS identification was further facilitated by the DNA microarray technology and bioinformatics, which is based on the previous experimental methods. The structural and functional properties of ARSs on yeast chromosomes are gradually explored in this field. In addition to the function of initiating replication, there is a growing interest for researchers in the effects of ARSs on gene silencing and expression of genes, particularly the relationship between ARSs and chromosome structure. In this review, we summarized the identification methods of ARSs, especially for the bioinformatics prediction methods over the past few years. The functions of ARSs are discussed in this research, moreover, ARS modification that combined with the high‐throughput sequencing was elaborated as well, shedding further light on the understanding of the roles of ARSs, and providing deep insights towards the optimization of ARSs.