Open AccessParallelized disruption of prokaryotic and eukaryotic cells via miniaturized and automated bead mill
Author(s) -
Jansen Roman P.,
Müller Moritz Fabian,
Schröter Sophie Edith,
Kappelmann Jannick,
Klein Bianca,
Oldiges Marco,
Noack Stephan
Publication year - 2020
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.202000002
Subject(s) - bioprocess , workflow , corynebacterium glutamicum , microfluidics , throughput , saccharomyces cerevisiae , proteomics , computational biology , biochemical engineering , biology , computer science , nanotechnology , bacteria , biochemistry , yeast , materials science , database , engineering , genetics , paleontology , telecommunications , gene , wireless
The application of integrated microbioreactor systems is rapidly becoming of more interest to accelerate strain characterization and bioprocess development. However, available high‐throughput screening capabilities are often limited to target extracellular compounds only. Consequently, there is a great demand for automated technologies allowing for miniaturized and parallel cell disruption providing access to intracellular measurements. In this study, a fully automated bead mill workflow was developed and validated for four different industrial platform organisms: Escherichia coli , Corynebacterium glutamicum , Saccharomyces cerevisiae , and Aspergillus niger . The workflow enables up to 48 parallel cell disruptions in microtiter plates and is applicable at‐line to running lab‐scale cultivations. The resulting cell extracts form the basis for quantitative omics studies where no rapid metabolic quenching is required (e.g., genomics and proteomics).