Open AccessImmobilization of formate dehydrogenase on polyethylenimine‐grafted graphene oxide with kinetics and stability study
Author(s) -
Lin Peng,
Zhang Yonghui,
Yao Guangxiao,
Huo Heyu,
Ren Hong,
Wang Yixuan,
Wang Shizhen,
Fang Baishan
Publication year - 2020
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201900134
Subject(s) - graphene , formate dehydrogenase , polyethylenimine , oxide , formate , materials science , chemistry , nanotechnology , combinatorial chemistry , organic chemistry , biochemistry , catalysis , transfection , gene
Graphene oxide‐based nanomaterials are promising for enzyme immobilization due to the possibilities of functionalizing surface. Polyethylenimine‐grafted graphene oxide was constructed as a novel scaffold for immobilization of formate dehydrogenase. Compared with free formate dehydrogenase and graphene oxide adsorbed formate dehydrogenase, thermostability, storage stability, and reusability of polyethylenimine‐grafted graphene oxide‐formate dehydrogenase were enhanced. Typically, polyethylenimine‐grafted graphene oxide‐formate dehydrogenase remained 47.4% activity after eight times’ repeat reaction. The immobilized capacity of the polyethylenimine‐grafted graphene oxide was 2.4‐folds of that of graphene oxide. Morphological and functional analysis of polyethylenimine‐grafted graphene oxide‐formate dehydrogenase was performed and the assembling mechanism based on multi‐level interactions was studied. Consequently, this practical and facile strategy will likely find applications in biosynthesis, biosensing, and biomedical engineering.