Open AccessA one‐pot process for synthesis of mitomycin analogs catalyzed by laccase/lipase optimized by response surface methodology
Author(s) -
Zhang Yuanyuan,
Yao Quancai,
Li Zewen,
Yang Fengke,
Wang Fanye,
Liu Junhong
Publication year - 2019
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201900118
Subject(s) - lipase , laccase , hydroquinone , yield (engineering) , catalysis , response surface methodology , chemistry , one pot synthesis , molar ratio , benzoquinone , organic chemistry , nuclear chemistry , chromatography , enzyme , materials science , metallurgy
To reach the excellent yield as well as environmental friendliness, an efficient one‐pot process for the synthesis of 2‐methyl‐3‐ n‐ butylaminoyl‐1,4‐benzoquinone, a mitomycin‐like compound by the domino reaction of 2‐methyl‐1,4‐hydroquinone and butylamine using laccase/lipase as co‐catalysts, has been developed. In this present study, the process proposed here was further improved by optimizing the relevant factors using the response surface methodology based on Box–Benkhen Design. The optimum condition that afforded the highest yield (98%) of 2‐methyl‐3‐ n‐ butylaminoyl‐1,4‐benzoquinone was obtained as follows: molar ratio of amines to hydroquinones 1.16:1, activity ratio of laccase to lipase 1.14:2, and reaction temperature 38.9°C. The results obtained indicate that this process may be useful as a green alternative method for higher yield production of mitomycin analogs.