Open AccessCharacterization and potential antitumor effect of a heteropolysaccharide produced by the red alga Porphyridium sordidum
Author(s) -
Nikolova Biliana,
Semkova Severina,
Tsoneva Iana,
Antov Georgi,
Ivanova Juliana,
Vasileva Ivanina,
Kardaleva Proletina,
Stoineva Ivanka,
Christova Nelly,
Nacheva Lilyana,
Kabaivanova Lyudmila
Publication year - 2019
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201900019
Subject(s) - polysaccharide , cancer cell , extracellular , galactose , fucoidan , biochemistry , biology , cell , electroporation , cancer , microbiology and biotechnology , pharmacology , genetics , gene
Abstract Taking into account the rising trend of the incidence of cancers of various organs, effective therapies are urgently needed to control human malignancies. However, almost all chemotherapy drugs currently on the market cause serious side effects. Fortunately, several studies have shown that some non‐toxic biological macromolecules, including algal polysaccharides, possess anti‐cancer activities or can increase the efficacy of conventional chemotherapy drugs. Polysaccharides are characteristic secondary metabolites of many algae. The efficacy of polysaccharides on the normal and cancer cells is not well investigated, but our investigations proved a cell specific effect of a newly isolated extracellular polysaccharide from the red microalga Porphyridium sordidum . The investigated substance was composed of xylose:glucose and galactose:manose:rhamnose in a molar ratio of 1:0.52:0.44:0.31. Reversible electroporation has been exploited to increase the transport through the plasma membrane into the tested breast cancer tumor cells MCF‐7 and MDA‐MB231. Application of 75 µg/mL polysaccharide in combination with 200 V/cm electroporation induced 40% decrease in viability of MDA‐MB231 cells and changes in cell morphology while control cells (MCF10A) remained with normal morphology and kept vitality.