Open AccessInduction and genetic identification of a callus‐like growth developed in the brown alga Fucus vesiculosus
Author(s) -
Zayed Ahmed,
Kovacheva Mariya,
Muffler Kai,
Breiner HansWerner,
Stoeck Thorsten,
Ulber Roland
Publication year - 2019
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201800211
Subject(s) - axenic , callus , fucus vesiculosus , biology , botany , brown algae , algae , bioproducts , bacteria , microbiology and biotechnology , biofuel , genetics
Abstract Induction of an axenic filamentous‐like callus growth from the brown algae Fucus vesiculosus is described. Different treatments were investigated in various combinations to develop axenic cultures based on identification of surface symbionts via 18S ribosomal RNA. Moreover, viability was confirmed after such processes by 2,3,5‐triphenyl tetrazolium chloride assay that demonstrated an average viability of 29%, relative to nonsterilized explants. After six weeks of a phototrophic cultivation on artificial sea water‐12‐nitrilotriacetic acid (0.5% w/v agar), a filamentous‐like callus growth was observed, which was identified genetically through its mitochondrial DNA after subculturing. Achievement of confirmed marine callus cultures might enrich old previously established blue biotechnology techniques and open new chances for cultivation of brown algae for production of good manufacturing practice‐compliant bioproducts.