Open AccessContinuous enzymatic production and adsorption of laminaribiose in packed bed reactors
Author(s) -
Abi Akram,
Hartig Dave,
Vorländer Karl,
Wang Anqi,
Scholl Stephan,
Jördening HansJoachim
Publication year - 2019
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201800110
Subject(s) - adsorption , chemistry , chromatography , packed bed , downstream processing , desorption , zeolite , sugar , biochemistry , organic chemistry , catalysis
Bienzymatic production of laminaribiose from sucrose and glucose was combined with adsorption on zeolite BEA to introduce a first capture and purification step. Downstream processing including washing and desorption steps was characterized and optimized on a milliliter scale in batch mode. Results were then transferred to a packed bed system for enzymatic production and adsorption where the influence of adsorbent particle diameter on purity and productivity was evaluated. Finally, a continuous enzymatic production of laminaribiose was conducted over 10 days. The subsequent downstream processing of the loaded zeolites led to purities of over 0.5 g Laminaribiose g sugar −1 in the desorbate with a total productivity of 5.6 mg Laminaribiose L enzyme bed −1 h −1 without the use of recycles.