Open AccessAn electrochemical biosensor for prostate cancer biomarker detection using graphene oxide–gold nanostructures
Author(s) -
Akbari jonous Zahra,
Shayeh Javad Shabani,
Yazdian Fatemeh,
Yadegari Amir,
Hashemi Mohadeseh,
Omidi Meisam
Publication year - 2019
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201800093
Subject(s) - biosensor , dielectric spectroscopy , materials science , graphene , detection limit , cyclic voltammetry , colloidal gold , electrode , electrochemistry , prostate specific antigen , nanotechnology , nanoparticle , analytical chemistry (journal) , chemistry , chromatography , prostate cancer , cancer , medicine
In this paper, a most sensitive electrochemical biosensor for detection of prostate‐specific antigen (PSA) was designed. To reach the goal, a sandwich type electrode composed of reduced graphene oxide/ gold nanoparticles (GO/AuNPs), Anti‐Total PSA monoclonal antibody, and anti‐Free PSA antibody was assembled. The functionalized materials were thoroughly characterized by atomic force microscope spectroscopy, transmission electron microscopy, and X‐ray diffraction techniques. The electrochemical properties of each of the modification step were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The results presented that the proposed biosensor possesses high sensitivity toward total and free PSA. Furthermore, the fabricated biosensor revealed an excellent selectivity for PSA in comparison to the other tumor markers such as BHCG, Alb, CEA, CA125, and CA19‐9. The limit of detection for the proposed electrochemical biosensor was estimated to be around 0.2 and 0.07 ng/mL for total and free PSA antigen, respectively.