Open AccessCell‐free production of pore forming toxins: Functional analysis of thermostable direct hemolysin from Vibrio parahaemolyticus
Author(s) -
Dondapati Srujan Kumar,
Wüstenhagen Doreen A,
Strauch Eckhard,
Kubick Stefan
Publication year - 2018
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201600259
Subject(s) - vibrio parahaemolyticus , hemolysin , bilayer , lipid bilayer , chemistry , conductance , biophysics , toxin , microbiology and biotechnology , biochemistry , bacteria , membrane , biology , gene , virulence , genetics , mathematics , combinatorics
Abstract The pore forming characteristic of TDH1 and TDH2 variants of thermostable direct hemolysin (TDH), a major toxin involved in the pathogenesis of Vibrio parahaemolyticus , was studied on a planar lipid bilayer painted over individual picoliter cavities containing microelectrodes assembled in a multiarray. Both proteins formed pores upon insertion into the lipid bilayer which was shown as a shift in the conductance from the baseline current. TDH2 protein was able to produce stable currents and the currents were influenced by external factors like concentration, type of salt and voltage. The pore currents were influenced and showed a detectable response in the presence of polymers which makes them suitable for biotechnology applications.