Open AccessFormulation of organic and inorganic hydrogel matrices for immobilization of β‐glucosidase in microfluidic platform
Author(s) -
Kazan Aslihan,
Heymuth Marcel,
Karabulut Dilan,
Akay Seref,
YildizOzturk Ece,
Onbas Rabia,
Muderrisoglu Cahit,
Sargin Sayit,
Heils Rene,
Smirnova Irina,
YesilCeliktas Ozlem
Publication year - 2017
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201600218
Subject(s) - self healing hydrogels , microreactor , tetraethyl orthosilicate , immobilized enzyme , chemical engineering , polyethylene , chemistry , microfluidics , silicon dioxide , biocatalysis , materials science , catalysis , chromatography , polymer chemistry , nanotechnology , organic chemistry , enzyme , ionic liquid , engineering
The aim of this study was to formulate silica and alginate hydrogels for immobilization of β‐glucosidase. For this purpose, enzyme kinetics in hydrogels were determined, activity of immobilized enzymes was compared with that of free enzyme, and structures of silica and alginate hydrogels were characterized in terms of surface area and pore size. The addition of polyethylene oxide improved the mechanical strength of the silica gels and 68% of the initial activity of the enzyme was preserved after immobilizing into tetraethyl orthosilicate–polyethylene oxide matrix where the relative activity in alginate beads was 87%. The immobilized β‐glucosidase was loaded into glass–silicon–glass microreactors and catalysis of 4‐nitrophenyl β‐ d ‐glucopyranoside was carried out at various retention times (5, 10, and 15 min) to compare the performance of silica and alginate hydrogels as immobilization matrices. The results indicated that alginate hydrogels exhibited slightly better properties than silica, which can be utilized for biocatalysis in microfluidic platforms.