Open AccessNovel approach for the prediction of cell densities and viability in standardized translucent cell culture biochips with near infrared spectroscopy
Author(s) -
Gröger Marko,
Lange Matthias,
Rennert Knut,
Kaschowitz Tobias,
Plettenberg Holger,
Hoffmann Martin,
Mosig Alexander S.
Publication year - 2017
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201600162
Subject(s) - biochip , microfluidics , viability assay , spectroscopy , cell culture , materials science , nanotechnology , cell , biomedical engineering , biological system , chemistry , biology , engineering , physics , biochemistry , genetics , quantum mechanics
Near infrared spectroscopy is a rapid and nondestructive method for compositional analysis of biological material. The technology is widely used within bioreactors and possesses potential as a standardized method for quality control in miniaturized microfluidic cell culture systems. Here, we established a method for quantification of cell density and viability of adherent HepaRG cells cultured in a translucent, miniaturized cell culture biochip. The newly developed statistical models for interpretation of near infrared spectroscopy from biochips are the basis for a novel method of fast, continuous, and contact‐free analysis of cell viability and real‐time monitoring of cell growth. The technique thus paves the way for a robust and reliable high‐throughput analysis of biochip‐embedded cell cultures.