Open AccessTowards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration
Author(s) -
Tosstorff Andreas,
Kroner Cora,
Opperman Diederik J.,
Hollmann Frank,
Holtmann Dirk
Publication year - 2017
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201600158
Subject(s) - cofactor , enzyme , process (computing) , regeneration (biology) , chemistry , stereochemistry , biochemistry , biology , computer science , microbiology and biotechnology , operating system
Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.