Open AccessMetabolic response of Pseudomonas putida to increased NADH regeneration rates
Author(s) -
Zobel Sebastian,
Kuepper Jannis,
Ebert Birgitta,
Wierckx Nick,
Blank Lars M.
Publication year - 2017
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201600072
Subject(s) - pseudomonas putida , formate dehydrogenase , formate , nad+ kinase , catabolism , biochemistry , metabolic engineering , cofactor , flux (metallurgy) , dehydrogenase , biology , metabolism , chemistry , enzyme , organic chemistry , catalysis
Pseudomonas putida efficiently utilizes many different carbon sources without the formation of byproducts even under conditions of stress. This implies a high degree of flexibility to cope with conditions that require a significantly altered distribution of carbon to either biomass or energy in the form of NADH. In the literature, co‐feeding of the reduced C1 compound formate to Escherichia coli heterologously expressing the NAD + ‐dependent formate dehydrogenase of the yeast Candida boidinii was demonstrated to boost various NADH‐demanding applications. Pseudomonas putida as emerging biotechnological workhorse is inherently equipped with an NAD + ‐dependent formate dehydrogenase encouraging us to investigate the use of formate and its effect on P. putida ’s metabolism. Hence, this study provides a detailed insight into the co‐utilization of formate and glucose by P. putida . Our results show that the addition of formate leads to a high increase in the NADH regeneration rate resulting in a very high biomass yield on glucose. Metabolic flux analysis revealed a significant flux rerouting from catabolism to anabolism. These metabolic insights argue further for P. putida as a host for redox cofactor demanding bioprocesses.