Open AccessDecolorizing an anthraquinone dye by Phlebia brevispora : Intra‐species characterization
Author(s) -
Lee Aslan Hwanhwi,
Jang Yeongseon,
Kim GyuHyeok,
Kim JaeJin,
Lee SungSuk,
Ahn ByoungJun
Publication year - 2017
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201600059
Subject(s) - laccase , anthraquinone , manganese peroxidase , chemistry , lignin peroxidase , anthracene , incubation , nuclear chemistry , peroxidase , spectrophotometry , fungus , food science , botany , chromatography , enzyme , biochemistry , biology , organic chemistry
Remazol brilliant blue R (RBBR) is an anthraquinone dye derived from anthracene that is decolorized by a white rot fungus, Phlebia brevispora . Interestingly, P. brevispora produces two phenomena of yellowish and pinkish colors during the degradation of RBBR. Here, we characterized the decolorization of RBBR by P. brevispora . The fungus was significantly different between the two colors via UV spectrophotometry, and the morphology of the hyphae observed in the respective color culture was also entirely different. Moreover, both of the two ligninolytic enzymes, laccase and manganese‐dependent peroxidase (MnP), were remarkably stimulated in the yellowish culture at the beginning of the decolorization. It is possible that the RBBR decolorizing mechanism might be primarily related to the amount of laccase and MnP produced in the yellowish culture. Thus, the decolorized color may be rapidly estimated at initial period of incubation. In addition, GeneFishing technology revealed that two genes were differentially expressed in yellowish culture.