Open AccessFarnesol stimulates laccase production in Trametes versicolor
Author(s) -
Hu Jianhua,
Wang Feng,
Ma Anzhou,
Zhuang Guoqiang,
Liu Ying,
Lu Jingsong,
Guo Chen,
Liu Chunzhao
Publication year - 2016
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201500082
Subject(s) - laccase , farnesol , trametes versicolor , quorum sensing , extracellular , mycelium , bioreactor , chemistry , microbiology and biotechnology , biochemistry , biology , gene , enzyme , botany , virulence
Farnesol, a quorum‐sensing molecule, was used successfully to improve laccase production in submerged cultures of Trametes versicolor . At the optimal farnesol concentration of 60 μM added at the beginning of the culture, the extracellular laccase activity reached 629.3 U L −1 after 6 days of cultivation, which represented a 1.92‐fold increase relative to the control without farnesol treatment. The addition of farnesol resulted in an increase in the accumulation of H 2 O 2 and an increased expression of the laccase ( lac ) gene and the RhoA gene. The RhoA gene correlated with hyperbranched mycelia, which facilitated the secretion of the intracellular laccase. This study provides a basis for understanding the induction mechanism of farnesol for enhancing laccase production.