Open AccessCombination of high‐frequency ultrasound and UV radiation of excilamp for surface disinfection
Author(s) -
Tsenter Irina,
Matafonova Galina,
Batoev Valeriy
Publication year - 2015
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201500073
Subject(s) - sonication , aqueous solution , chemistry , spore , irradiation , materials science , microbiology and biotechnology , chromatography , biology , organic chemistry , physics , nuclear physics
The combination of high‐frequency ultrasound (HFUS) and UV represents a new approach to disinfecting surfaces. This study aimed to examine the inactivation efficiency of HFUS (1.7 MHz) and monochromatic UV radiation of KrCl excilamp (222 nm) in a single and a sequential mode against Bacillus cereus cells and spores added to glass surfaces. When treated by UV only, cells at populations of 10 3 , 10 4 , and 10 5 colony‐forming units (CFU)/cm 2 showed 100% disinfection at high doses up to 1760 mJ/cm 2 . Spores at 10 4 CFU/cm 2 were completely inactivated at a dose of 1170 mJ/cm 2 . Treatment with aqueous aerosol (produced by HFUS) reduced cell counts by 100% within a 40‐min exposure, whereas it was ineffective in inactivating spores under these conditions. In a sequential mode, the contaminated surface was pretreated with the sonicated aqueous aerosol and subsequently irradiated with the excilamp. It was found that HFUS exposure times and UV doses for complete inactivation decreased by a factor of 2 and 6–7, respectively, compared to sole HFUS or UV. A portable apparatus for surface disinfection was designed. The combined HFUS/UV method may be a promising technique for rapid disinfection of microbially contaminated surfaces.