Open AccessCalibration‐free inverse modeling of ion‐exchange chromatography in industrial antibody purification
Author(s) -
Hahn Tobias,
Huuk Thiemo,
Osberghaus Anna,
Doninger Katharina,
Nath Susanne,
Hepbildikler Stefan,
Heuveline Vincent,
Hubbuch Jürgen
Publication year - 2016
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201400248
Subject(s) - chromatography , calibration , process optimization , chemistry , process (computing) , biological system , nonlinear system , impurity , ion chromatography , inverse , computer science , mathematics , engineering , chemical engineering , physics , statistics , geometry , organic chemistry , quantum mechanics , biology , operating system
The identification of optimal process parameters for the isolation of a target component from multicomponent mixtures is especially challenging in industrial applications. With constantly increasing time‐to‐market pressure, screening a large parameter space is not feasible and design‐of‐experiment approaches with few experiments might fail due to dynamic and nonlinear reactions to small parameter changes. Model‐based optimization can determine optimal operating conditions, once the model has been calibrated to the specific process step. In this work, parameters for the steric mass action model were estimated for the target protein and three impurities of an industrial antibody cation‐exchange purification step using only chromatograms at different wavelengths and additional fraction analyses with size exclusion chromatography. Information on the molar or mass concentrations in the feed are not available. The model‐based optimization results coincide with conventional chromatogram‐based optimization.