Open AccessEngineering Streptomyces diastatochromogenes 1628 to increase the production of toyocamycin
Author(s) -
Tao Libin,
Ma Zheng,
Xu Xianhao,
Bechthold Andreas,
Bian Yalin,
Shentu Xuping,
Yu Xiaoping
Publication year - 2015
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201400239
Subject(s) - streptomyces , production (economics) , engineering , biochemical engineering , biology , genetics , economics , bacteria , macroeconomics
Toyocamycin (TM) shows antifungal activity and can be used to treat plant diseases. TM is produced by Streptomyces diastatochromogenes 1628, but the production rate is always very low. Recently, the gene toy G was cloned; however, nothing was known about the influence of toy G on TM production. In this study, the rational combined co‐expression of toy G with frr encoding a ribosome recycling factor and vgb encoding Vitreoscilla hemoglobin was performed. Introduction of these genes in double‐ or triple‐combination into strain S. diastatochromogenes 1628 led to greatly increased TM production. Transcription analysis revealed that higher TM production in the constructed recombinant strains resulted from an increase in transcriptional levels of toy G gene. At the end, among all these different recombinant strains, the strain S. diastatochromogenes 1628‐VGF, harboring vgb , frr , and toy G placed under the control of PermE* , exhibited the largest increase in TM production. Notably, TM produced by S. diastatochromogenes 1628‐VGF reached the highest level of 319.2 mg/L at 72 h, while TM yield of the original strain 1628 was 147.1 mg/L at 84 h. The high‐yield, energy‐saving strain 1628‐VGF constructed in this study shall be beneficial for the industrial production of TM.