Open AccessEngineering an industrial Saccharomyces cerevisiae strain with the inulinase gene for more efficient ethanol production from Jerusalem artichoke tubers
Author(s) -
Yuan WenJie,
Li NanNan,
Zhao XinQing,
Chen LiJie,
Kong Liang,
Bai FengWu
Publication year - 2013
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201200199
Subject(s) - jerusalem artichoke , inulinase , kluyveromyces marxianus , ethanol fuel , fermentation , saccharomyces cerevisiae , yeast , food science , bioprocess , ethanol , biology , ethanol fermentation , strain (injury) , metabolic engineering , biochemistry , chemistry , gene , anatomy , paleontology
Engineering industrial microbial strains with inulinase production for developing a consolidated bioprocessing (CBP) strategy is a desirable solution to biofuel production from Jerusalem artichoke tubers. In this study, the integrative vector pFA6a‐rDNA‐PGK‐INU was generated by fusing the 3.3‐kb ribosomal DNA fragment with the phosphoglycerate kinase promoter to regulate the expression of the inulinase gene isolated from Kluyveromyces marxianus , which was then integrated into the chromosome of an industrial Saccharomyces cerevisiae strain for ethanol production from Jerusalem artichoke tubers. Compared to the host strain, no significant difference was observed in the growth of the recombinant yeast, but its inulinase production was enhanced: 22.9 versus 10.6 U/mL for aerobic seed cultures and 14.5 versus 10.0 U/mL for ethanol fermentation, which consequently facilitated the CBP process for ethanol production: 72.5 g/L ethanol produced in a fermentation time of 48 h, while only 67.0 g/L ethanol was produced by the host strain in a fermentation time of 60 h. Thus, the ethanol productivity was increased from 1.12 to 1.51 g/L/h, presenting an increase of 34.8%.