Open AccessCandida rugosa lipase‐catalyzed synthesis of sucrose‐6‐acetate in a 2‐butanol/buffer two‐phase system
Author(s) -
Zhong Xiang,
Qian Junqing,
Liu Min,
Ma Lingzhi
Publication year - 2013
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201200170
Subject(s) - candida rugosa , lipase , butanol , sucrose , chemistry , buffer (optical fiber) , catalysis , n butanol , phase (matter) , chromatography , organic chemistry , enzyme , ethanol , telecommunications , computer science
Sucrose‐6‐acetate, a short‐chain monoester, is an important intermediate for the preparation of sucralose. Regioselective transformations of mono‐ and oligosaccharides by enzymes have been performed in organic solvents. The present study was carried out with Candida rugosa lipase to synthesize sucrose‐6‐acetate by transesterification of sucrose and vinyl acetate in a two‐phase system consisting of a buffer solution and an organic solvent. The screening results revealed that 2‐butanol/Tris‐HCl was the optimal two‐phase system for biocatalysis, and other factors influencing the synthesis of sucrose‐6‐acetate were also studied. Three major factors (temperature, initial buffer pH, and molar ratio of substrates) were identified through Plackett–Burman design, and the response surface methodology was employed to optimize the reaction process. Under optimal conditions, the yield of sucrose‐6‐acetate reached up to 57.42%. The aqueous two‐phase system was successfully applied to the synthesis of sucrose‐6‐acetate with high yield, low toxicity, good resolution, low cost, and simple scale‐up.