Open AccessImmobilization of cellulases on the reversibly soluble polymer E udragit S ‐100 for cotton treatment
Author(s) -
Yu Yuanyuan,
Yuan Jiugang,
Wang Qiang,
Fan Xuerong,
Wang Ping,
Sun Xuejiao
Publication year - 2013
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201200086
Subject(s) - cellulase , ultimate tensile strength , cellulose , polymer , covalent bond , hydrolysis , chemistry , size exclusion chromatography , materials science , fiber , filtration (mathematics) , chemical engineering , composite material , enzyme , organic chemistry , mathematics , engineering , statistics
Cellulases can penetrate into the fiber, causing tensile strength loss of the cellulosic fibers or fabrics. To minimize the tensile strength loss, we have immobilized cellulases on E udragit S ‐100. The characteristics of covalent E udragit cellulase were evaluated using gel filtration analysis and UV spectra. Gel filtration analysis revealed that the cellulases were covalently bound to the polymer. Covalent E udragit cellulase was loaded with the enzyme of about 40% and had a relative activity about 80% at a E udragit S ‐100 concentration of 15 g/L. When cellulase is bound to the polymer, the solubility profile becomes similar to the one of E udragit. In addition, the effects of the enzyme on the cotton yarns and fabric using cellulases have been investigated. Native and immobilized cellulases caused improvements in whiteness and wrinkle recovery angle of the fabric in comparison to the control samples. The bending stiffness results show that native and immobilized cellulase treated cotton fabric has an improved softness than the control samples. It was found that using the immobilized cellulase reduced the weight and tensile strength, because the hydrolytic attack is only limited to the surfaces of cotton fibers.