Open AccessSuccinic acid production from n ‐alkanes
Author(s) -
Kamzolova Svetlana V.,
Vinokurova Natalia G.,
Yusupova Alsu I.,
Morgunov Igor G.
Publication year - 2012
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201100241
Subject(s) - yarrowia , succinic acid , chemistry , hydrogen peroxide , yield (engineering) , residue (chemistry) , extraction (chemistry) , ethanol , ethyl acetate , thiamine , chromatography , yeast , hexane , butanol , organic chemistry , biochemistry , materials science , metallurgy
A novel process of production of succinic acid ( SA ) has been developed, which includes the synthesis of alpha‐ketoglutaric acid by a thiamine‐auxotrophic yeast strain Yarrowia lipolytica VKM Y‐2412 from n ‐alkanes and subsequent oxidation of the acid by hydrogen peroxide to SA . The concentration of SA in the culture broth and its yield were found to be 38.8 g/L and 82.45% of n ‐alkane consumed, respectively. The isolation procedure involved the extraction of the residual alkanes with the mixture of ethyl acetate and hexane, the decomposition of H 2 O 2 in the filtrate followed by filtrate bleaching and acidification with a mineral acid; the evaporation of filtrate and the ethanol extraction of SA from lyophilized residue. The purity of the SA isolated from the culture liquid filtrate reached 99.5%.