Open AccessEffect of temperature on ethanol tolerance of thermotolerant I sshatchenkia orientalis IPE 100
Author(s) -
Kwon YongJin,
Wang Feng,
Li Qian,
Liu ChunZhao
Publication year - 2013
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201100205
Subject(s) - ethanol , fermentation , yeast , chemistry , ethanol content , food science , ethanol fuel , product inhibition , ethanol fermentation , growth inhibition , biochemistry , cell growth , non competitive inhibition , enzyme
Tolerance to high temperature and ethanol is a major factor in high‐temperature bio‐ethanol fermentation. The inhibitory effect of exogenously added ethanol (0–100 g L −1 ) on the growth of the newly isolated thermotolerant I ssatchenkia orientalis IPE 100 was evaluated at a range of temperatures (30–45°C). A generalized M onod equation with product inhibition was used to quantify ethanol tolerance, and it correlated well with the experimental data on microbial growth inhibition of ethanol at the temperatures of 30–45°C. The maximum inhibitory concentration of ethanol for growth ( P m ) and toxic power ( n ) at the optimal growth temperature of 42°C were estimated to be 96.7 g L −1 and 1.23, respectively. The recently isolated thermotolerant I . orientalis IPE 100 shows therefore a strong potential for the development of future high‐temperature bio‐ethanol fermentation technologies. This study provides useful insights into our understanding of the temperature‐dependent inhibitory effects of ethanol on yeast growth.