Open AccessEffect of ionic liquids activation on laccase from T rametes versicolor : Enzymatic stability and activity
Author(s) -
Tavares Ana P. M.,
Pereira Juliana A. N.,
Xavier Ana M. R. B.
Publication year - 2012
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201100203
Subject(s) - laccase , ionic liquid , chemistry , trametes versicolor , kinetics , activation energy , reaction rate constant , enzyme , thermal stability , enzyme kinetics , nuclear chemistry , organic chemistry , catalysis , active site , physics , quantum mechanics
The stability and activity of laccase from T rametes versicolor in two water‐soluble ionic liquids ( IL s), namely 1‐butyl‐3‐methylimidazolium methyl sulfate, [bmim][MeSO 4 ] and 1,3‐dimethylimidazolium methyl sulfate, [mmim][MeSO 4 ], were investigated in this study. Thermal inactivation of laccase was characterized in the presence of these both IL s and as expected first‐order kinetics was followed. Inactivation rate constant ( k ), half‐life time ( t 1/2 ), and energy of activation ( Ea ) were determined. Kinetics of 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) oxidation by laccase in the presence of these IL s was studied and M ichaelis– M enten parameters were calculated. There is no enzymatic inactivation since the maximum reaction rate remained constant for IL concentrations up to 25%, and surprisingly, it was found that laccase was activated for concentrations of 35% of IL s, since the reaction rate increased 1.7 times.