Open AccessGalanthamine production by L eucojum aestivum L . shoot culture in a modified bubble column bioreactor with internal sections
Author(s) -
Georgiev Vasil,
Ivanov Ivan,
Berkov Strahil,
Ilieva Mladenka,
Georgiev Milen,
Gocheva Tatiana,
Pavlov Atanas
Publication year - 2012
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201100177
Subject(s) - shoot , bioreactor , lycorine , botany , dry weight , chlorophyll , chlorophyll a , chromatography , amaryllidaceae alkaloids , biology , murashige and skoog medium , chemistry , horticulture , amaryllidaceae , tissue culture , alkaloid , biochemistry , in vitro
Shoot culture of summer snowflake ( L eucojum aestivum L .) was successfully cultivated in an advanced modified glass‐column bioreactor with internal sections for production of Amaryllidaceae alkaloids. The highest amounts of dry biomass (20.8 g/L) and galanthamine (1.7 mg/L) were achieved when shoots were cultured at 22°C and 18 L/(L·h) flow rate of inlet air. At these conditions, the L . aestivum shoot culture possessed mixotrophic‐type nutrition, synthesizing the highest amounts of chlorophyll (0.24 mg/g DW (dry weight) chlorophyll A and 0.13 mg/g DW chlorophyll B ). The alkaloids extract of shoot biomass showed high acetylcholinesterase inhibitory activity ( IC 50 = 4.6 mg). The gas chromatography–mass spectrometry (GC/MS) profiling of biosynthesized alkaloids revealed that galanthamine and related compounds were presented in higher extracellular proportions while lycorine and hemanthamine‐type compounds had higher intracellular proportions. The developed modified bubble‐column bioreactor with internal sections provided conditions ensuring the growth and galanthamine production by L . aestivum shoot culture.