Open AccessPolyhydroxybutyrate production by Saccharophagus degradans using raw starch as carbon source
Author(s) -
GonzálezGarcía Yolanda,
Rosales Mario A.,
GonzálezReynoso Orfil,
SanjuánDueñas Rubén,
Córdova Jesús
Publication year - 2011
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201000118
Subject(s) - starch , polyhydroxybutyrate , biopolymer , carbon source , food science , raw material , carbon fibers , chemistry , polyhydroxyalkanoates , polysaccharide , potato starch , bacteria , biochemistry , chemical engineering , materials science , organic chemistry , polymer , biology , genetics , composite number , composite material , engineering
Biosynthesis of poly(3‐hydroxybutyrate) (PHB) from raw starch as the carbon source by the polysaccharide‐digesting bacteria Saccharophagus degradans was investigated in a fed‐batch culture. The production and properties of the PHB synthesized from starch were compared to those obtained using glucose as carbon source. In fed‐batch cultures, S. degradans accumulated 21.35 and 17.46% of PHB, using glucose or starch as carbon source, respectively. The physical properties of the biopolymer produced from each carbon source were similar between them. Molecular mass, melting temperature and heat of fusion were 54.23 kDa, 165.61°C and 59.59 J/g, respectively, using glucose; and 57.07 kDa, 174.31°C and 67.66 J/g, respectively, using starch. This is the first work describing the capability of S. degradans to utilize raw starch as the sole carbon source for the production of PHB.