Open AccessMicrofluidic devices and true‐color sensor as platform for glucose oxidase and laccase assays
Author(s) -
Demirkol Dilek Odaci,
Dornbusch Kay,
Feller KarlHeinz,
Timur Suna
Publication year - 2011
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201000068
Subject(s) - laccase , repeatability , glucose oxidase , abts , chemistry , microfluidics , chromatography , biosensor , enzyme , biochemistry , nanotechnology , materials science , antioxidant , dpph
The commonly used assay for measuring glucose oxidase (GOx) and laccase (Lac) activity, based on spectrophotometric test using 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), has been adapted to measure these enzyme activities via microfluidic devices. The effects of flow rate and concentration of substrates were examined. The developed assays allowed analyzing GOx and Lac activities in the range of 0.8–8.0 Unit (0.1–1.0 mg/mL) and 0.9–17.7 Unit (0.1–2.0 mg/mL), respectively. Moreover, repeatability tests were obtained for both enzyme assays.