Open AccessDevelopment of emulsion from rhizobial fermented starch industry wastewater for application as Medicago sativa seed coat
Author(s) -
John Rojan Pappy,
Tyagi Rajeshwar Dayal,
Brar Satinder Kaur,
Prévost Danielle
Publication year - 2010
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.201000002
Subject(s) - emulsion , sinorhizobium meliloti , food science , starch , xanthan gum , canola , chemistry , inoculation , sowing , biology , horticulture , bacteria , biochemistry , rheology , materials science , symbiosis , composite material , genetics
Starch industry wastewater was efficiently employed for the production of Sinorhizobium meliloti and the concentrated culture was used for the development of a biofertilizer formulation. Tween‐80 (0.02 g/L) acted as the best emulsifier for a Sinorhizobium –canola oil emulsion. The stability of the emulsion and survival of the organism was enhanced by supplementation of xanthan gum at pH 8. The refrigerated condition was most favorable for stability and survival of the microorganism. The survival of microorganism at 4±1°C was 2.78×10 10 and 2.01×10 10 CFU (colony forming unit)/mL on storage for 1 and 2 months, respectively. The values were higher than the prescribed cell count (×10 3 CFU/mL) for field application. At 40°C, the survival of bacteria reduced from 3×10 10 CFU/mL to 8.1×10 9 and 8.8×10 6 CFU/mL in 1 and 2 months, respectively. Emulsion‐coated seed was incubated at different temperatures and a cell count of 10 5 CFU/seed was observed after 2 months of storage at 4°C, which was equal to the highest level of the described requirement (10 3 –10 5 CFU/seed). Emulsion supplemented with xanthan gum improved the shelf‐life under optimized conditions ( Sinorhizobium concentrate – canola oil (1:1) emulsion with 0.02 g/L Tween‐80; storage at pH 8 and temperature 4±1°C) and this emulsion with the required cell count and prolonged viability was used for the pre‐inoculation of seed or for in situ soil application.