Open AccessLaccase production from oil palm industry solid waste: Statistical optimization of selected process parameters
Author(s) -
Annuar M. Suffian M.,
Murthy Sangeetha Sammantha,
Sabanatham Vikineswary
Publication year - 2010
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200900044
Subject(s) - laccase , solid state fermentation , tray , fermentation , moisture , bioreactor , pulp and paper industry , nitrogen , materials science , chemistry , urea , botany , food science , composite material , biochemistry , biology , engineering , organic chemistry , enzyme
Oil palm frond parenchyma tissue was used as a solid substrate for the production of laccase via solid‐state fermentation using the white rot fungus Pycnoporus sanguineus . With a rectangular aluminium tray as solid‐state fermentation bioreactor, process parameters such as bed height, moisture and supplemented nitrogen (as urea solution) levels were studied and optimized using a statistical design of experiment. The moisture level exerted a significant effect on the process. The interaction effect observed between bed height and supplemented nitrogen level suggested that uniform distribution of supplemented nitrogen into the substrate bed was important. The proposed regression model sufficiently predicted the process response over the experimental range tested. The optimum parameter combination for laccase production was a 3‐cm bed height, 72% w/w moisture and 0.21% w/v supplemented nitrogen. Laccase productivity remained constant when the tray size was increased from 1.4 to 3.4‐fold.