Open AccessImmobilization of acetylcholinesterase in nanofibrous PVA/BSA membranes by electrospinning
Author(s) -
Moradzadegan Atousa,
RanaeiSiadat SeyedOmid,
EbrahimHabibi Azadeh,
BarshanTashnizi Mohammad,
Jalili Rouhollah,
Torabi SeyedFakhraddin,
Khajeh Khosro
Publication year - 2010
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200900001
Subject(s) - electrospinning , nanofiber , acetylcholinesterase , immobilized enzyme , membrane , chemistry , aché , chromatography , enzyme assay , enzyme , chemical engineering , polymer chemistry , materials science , nanotechnology , polymer , organic chemistry , biochemistry , engineering
Electrospinning, a simple and versatile method to fabricate nanofibrous supports, has attracted continuous attention in the field of enzyme immobilization. In this study, acetylcholinesterase (AChE) has been successfully immobilized in PVA nanofibers via electrospinning of a mixture of AChE, BSA as an enzyme stabilizing additive and PVA. The maximum activity recovery of immobilized AChE was about 40%. In comparison with free enzyme, the immobilized AChE showed improved stability while retaining a considerable amount of activity at lower pH values. Moreover, the immobilized AChE retained >34% of its initial activity when stored at 30°C for 100 days and retained 70% of its initial activity after ten consecutive reactor batch cycles.