Open AccessScale up production of isoflavonoids in cell suspension cultures of Pueraria tuberosa grown in shake flasks and bioreactor
Author(s) -
Sharma Varsha,
Goyal Shaily,
Ramawat Kishan Gopal
Publication year - 2009
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/elsc.200800114
Subject(s) - pueraria , isoflavonoid , bioreactor , suspension culture , chemistry , yield (engineering) , food science , laboratory flask , botany , biology , flavonoid , cell culture , biochemistry , materials science , medicine , alternative medicine , genetics , pathology , metallurgy , antioxidant
Cell cultures of Pueraria tuberosa were grown in vessels of different sizes and 2L stirred tank bioreactor containing modified MS medium with morphactin (0.1 mg l −1 ) and 2iP (5.0 mg l −1 ) and 20% inoculum. Stable growth and total isoflavonoid yield of 76.6 mg l −1 were recorded in the cultures during scale up. This was in concordance with the persistent yield of the individual isoflavonoids regardless of the vessel size.